Journal
FEBS JOURNAL
Volume 272, Issue 1, Pages 282-289Publisher
WILEY
DOI: 10.1111/j.1432-1033.2004.04406.x
Keywords
CENP-B; centromere; DNA methylation; chromatin; heterochromatin
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In eukaryotes, CpG methylation is an epigenetic DNA modification that is important for heterochromatin formation. Centromere protein B (CENP-B) specifically binds to the centromeric 17 base-pair CENP-B box DNA, which contains two CpG dinucleotides. In this study, we tested complex formation by the DNA-binding domain of CENP-B with methylated and unmethylated CENP-B box DNAs, and found that CENP-B preferentially binds to the unmethylated CENP-B box DNA. Competition analyses revealed that the affinity of CENP-B for the CENP-B box DNA is reduced nearly to the level of nonspecific DNA binding by CpG methylation.
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