Journal
CHEMICAL COMMUNICATIONS
Volume -, Issue 27, Pages 3484-3486Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b505744b
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The resolution of asparagine-type oligosaccharides in glycoproteins was carried out by combination of the transglycosylation reaction and CE-TOF-MS. The oligosaccharides enzymatically transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M. The resulting fluorescent-oligosaccharides were separated by CE and detected by TOF-MS. Disialo-Asn was successfully identified by the proposed procedure. Application to oligosaccharides in ovalbumin was also described in this communication.
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