4.6 Article

Infrared imaging of calcified tissue in bone biopsies from adults with osteomalacia

Journal

BONE
Volume 36, Issue 1, Pages 6-12

Publisher

ELSEVIER SCIENCE INC
DOI: 10.1016/j.bone.2004.08.019

Keywords

bone size and geometry; osteomalacia; infrared imaging; mineral content; crystallinity; collagen

Funding

  1. NCRR NIH HHS [C06 RR012538-01] Funding Source: Medline
  2. NIAMS NIH HHS [AR046121, AR041325, R01 AR041325-12] Funding Source: Medline
  3. NATIONAL CENTER FOR RESEARCH RESOURCES [C06RR012538] Funding Source: NIH RePORTER
  4. NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR041325, P30AR046121] Funding Source: NIH RePORTER

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Osteomalacia is a pathological bone condition in which there is deficient primary mineralization of the matrix, leading to an accumulation of osteoid tissue and reduced bone mechanical strength. The hypothesis that there are no qualitative or quantitative differences in osteomalacic bone mineral or matrix compared to disease-free bones was tested by examining unstained sections of polymethyl methacrylate (PMMA) embedded iliac crest biopsies using Fourier transform infrared imaging (FTIRI) at approximately 6-mum spatial resolution. Controls were seven female subjects, aged 36-57, without apparent bone disease. The experimental group consisted of I I patients aged 22-72, diagnosed with osteomalacia. The spectroscopic parameters analyzed in each data set were previously established as sensitive to bone quality: phosphate/amide 1 band area ratio (mineral content), 1660/1690 cm(-1) peak ratio (collagen cross-links), and the 1030/1020 cm(-1) peak ratio (mineral crystallinity). The correspondence between spectroscopic mineral content (phosphate/amide 1 ratio) and ash weight was validated for apatite crystals of different composition and crystallite size. The FTIRI results from the biopsies expressed as color-coded images and pixel population means were compared with the nonparametric Mann-Whitney U test. There were no significant differences in the cortical parameters. Significant difference was found in the mineral content of the trabecular regions with a lower mean value in osteomalacia (P = 0.01) than in controls. Mineral crystallinity tended to be decreased in the trabecular bone (P = 0.09). This study supports the hypothesis that, in osteomalacia, the quality of the organic matrix and of mineral in the center of bone does not change, while less-than-optimal mineralization occurs at the bone surface. This study provides the first spectroscopic evaluation of whole bone mineral and matrix properties in osteomalacia, demonstrating that there are few differences in collagen cross-links between biopsies from patients with osteomalacia and from individuals without histological evidence of bone disease. (C) 2004 Elsevier Inc. All rights reserved.

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