Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 3, Issue 15, Pages 2688-2690Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b506155e
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- Wellcome Trust Funding Source: Medline
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Cytochrome P450(BM3), from Bacillus megaterium, catalyses the epoxidation of linolenic acid 1 yielding 15,16epoxyoctadeca-9,12-dienoic acid 2 with complete regio-and moderate enantio-selectivity (60% ee). The absolute configuration of the product is tentatively assigned as 15(R), 16(S)-. The Michaelis-Menten parameters k(cat) and Km for the reaction were determined to be 3126 +/- 226 min(-1) 1 and 24 +/- 6 mu M respectively.
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