Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 3, Issue 6, Pages 1002-1007Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b418078j
Keywords
-
Categories
Ask authors/readers for more resources
We have designed a second-generation TASC (target- assisted self-cleavage) probe. It is based on the switching-on of incorporated cis- acting DNAzyme activity upon the target- induced conformational change of the otherwise inactive off-target probes locked in an intrastrand base- paired hairpin geometry. With E. coli 16S ribosomal RNA-relevant oligonucleotides as targets, the locked TASC probe exhibits an allosteric factor of k(on)/k(off) = 65 and the sequence selectivity is high, in terms of single nucleotide difference, when particular sequence and length of targets are chosen. Preliminary experiments with fixed E. coli cells show that the locked TASC probe with a FRET pair can be used to image fixed E. coli cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available