Regulation of branchial V-H+-ATPase Na+/K+-ATPase and NHE2 in response to acid and base infusions in the Pacific spiny dogfish (Squalus acanthias)

To study the mechanisms of branchial acid-base regulation, Pacific spiny dogfish were infused intravenously for 24h with either HCI (495 +/- 79 mumol kg(-1)h(-1)) or NaHCO3 (981 +/- 235 mumol kg(-1) h(-1)). Infusion of HCI produced a transient reduction in blood pH. Despite continued infusion of acid, pH returned to normal by 12 h. Infusion of NaHCO3 resulted in a new steady-state acid-base status at similar to0.3 pH units higher than the controls. Immunostained serial sections of gill revealed the presence of separate vacuolar proton ATPase (Na+/K+-ATPase)-rich or sodium-potassium ATPase (Na+/K+-ATPase)-rich cells in all fish examined. A minority of the cells also labeled positive for both transporters. Gill cell membranes prepared from NaHCO3-infused fish showed significant increases in both V-H+-ATPase abundance (300 +/- 81%) and activity. In addition, we found that V-H(+)ATPase subcellular localization was mainly cytoplasmic in control and HO-infused fish, while NaHCO3-infused fish demonstrated a distinctly basolateral staining pattern. Western analysis in gill membranes from HO-infused fish also revealed increased abundance of Na+/H+ exchanger 2 (213 +/- 5%) and Na+/K+-ATPase (315+/-88%) compared to the control.