Journal
JOURNAL OF ENVIRONMENTAL MANAGEMENT
Volume 162, Issue -, Pages 74-80Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jenvman.2015.07.039
Keywords
Hospital wastewaters; Ozonolysis; Sonolysis; Plasmid; DNA denaturation
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Funding
- Universidade do Vale do Itajai (Itajai, SC, Brazil)
- CNPq (Brazilian National Council for Scientific and Technological Development) [470640/2007-3]
- CNPq [300645/2010-4, 140452/2010-9]
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Hospital wastewater treatments must ensure that all genetic material is destroyed, since nuclear and extra-nuclear DNA can show antimicrobial resistance and contain recombinant genes, which promote vertical and/or horizontal gene transfer, amplifying the current problem of the emergence of antibiotic-resistant microorganisms. In this study, we investigated whether ozonolysis or ozonolysis/sonolysis in combination can denature genetic material, i.e., destroy the integrity of DNA molecules, present in hospital wastewaters. To achieve this goal, hospital wastewaters were treated by ozonolysis or ozonolysis/sonolysis in combination (at 70 and 100 W L-1) and both raw and treated wastewaters were analyzed in terms of disinfection and DNA denaturation efficiency quantified by viable cell counts and by agarose gel electrophoresis. In the ozonolysis treatment, the agarose gel electrophoresis technique showed that the ozone-treated samples contained DNA molecules, while combined ozonolysis/sonolysis destroyed the DNA in a power density-dependent manner (64% at 70W L-1 and 81% at 100 W L-1). Care must be taken by environmental managers to distinguish disinfection processes from DNA denaturation processes, since these two terms are not synonymous. (C) 2015 Elsevier Ltd. All rights reserved.
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