The inactivation of phospholipase A(2) by scalaradial: a biomimetic study by electrospray mass spectrometry

A biomimetic approach was employed to shed light on the nature of chemical reactions occurring in the covalent inactivation of phospholipase A(2) (PLA(2)) by scalaradial (1), a marine dialdehyde terpenoid endowed with potent anti-inflammatory activity. To this end, a detailed study of the reaction profile between the nitrogenous nucleophile isopropylamine and scalaradial was performed under biologically relevant conditions. Copyright (C) 2005 John Wiley Sons, Ltd.