Journal
ELECTROPHORESIS
Volume 26, Issue 1, Pages 53-59Publisher
WILEY
DOI: 10.1002/elps.200406088
Keywords
ampholytic dyes; pl markers
Ask authors/readers for more resources
Newly prepared azo compounds and several commercially available indicators were investigated for their applicability as colored isoelectric point (pl) markers for isoelectric focusing (IEF) in the acidic range below pH 5. The majority of compounds described here can serve as primary standards since their p/ values were determined by UV-VIS spectrophotometry independently IEF and direct measurement with a pH electrode. Subjected to gel JEF they show narrow and well-observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of p/ markers covering the pH range from 1.5 to 4.7.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available