Journal
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
Volume 69, Issue 1, Pages 255-257Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1271/bbb.69.255
Keywords
amino acid analysis; tryptophan; hydrolyis; protein
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The acid hydrolysis of proteins was miniaturized and simplified by employing microcapillary tubes (100 proportional tol in volume) with 6 m HCI containing 1% 2-mercaptoethanol and 3% phenol for an amino acid compositional analysis. The method not only eliminated the laborious evacuation step for the hydrolysis tube but also decreased the destruction of tryptophan during hydrolysis. The recovery of tryptophan was 79% by acid hydrolysis at 145degreesC for 4 h. Since the acid mixture could be removed under vacuum, the hydrolysate was subjected to an amino acid analysis without neutralization or dilution.
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