Journal
BIOTECHNOLOGY LETTERS
Volume 27, Issue 1, Pages 7-11Publisher
SPRINGER
DOI: 10.1007/s10529-004-6300-3
Keywords
N-acetyl-D-glucosamine; Aeromonas sp; GJ-18; chitinolytic activity; N,N '-diacetylchitobiose
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A bacterium, Aeromonas sp. GJ-18, having strong chitinolytic activity was isolated from coastal soil and used for crude enzyme preparations. This enzyme preparation contained N-acetyl-D-glucosaminidase and N,N'-diacetylchitobiohydrolase. N-Acetyl-D-glucosaminidase was inactive above 50 degreesC, but N,N'-diacetylchitobiohydrolase was stable at this temperature. Utilizing the temperature sensitivities of the chitin degradation enzymes in crude enzyme preparation,N-acetyl-D-glucosamine (GlcNAc) and N, N'-diacetylchitobiose [(GlcNAc)(2)] were selectively produced from chitin. At 45 degreesC, GlcNAc was produced as a major hydrolytic product (94% composition) with a yield of 74% in 5 d, meanwhile at 55 degreesC ( GlcNAc) 2 was the major product (86%) with a yield of 35% within 5 d.
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