Journal
PLANT JOURNAL
Volume 41, Issue 1, Pages 133-145Publisher
WILEY
DOI: 10.1111/j.1365-313X.2004.02284.x
Keywords
after-ripening; coat-imposed dormancy; beta-1,3-glucanase; gene expression; low-hydrated state; seed germination; testa rupture
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An air-dry developmental state with low-hydrated tissues is a characteristic of most plant seeds. Seed dormancy is an intrinsic block of germination and can be released during after-ripening, that is air-dry storage of mature seeds. Both seed-covering layers, testa and endosperm, cause the coat-imposed dormancy of tobacco (Nicotiana tabacum). After-ripening and over-expression of class I beta-1,3-glucanase (betaGlu I) confer maternal effects on testa rupture and dormancy release. Very little is known about the molecular mechanisms of after-ripening and whether gene expression is possible in low-hydrated seeds. Transient, low-level betaGlu I transcription and translation was detected during tobacco seed after-ripening. H-1 NMR 2D micro-imaging showed uneven distribution of proton mobility in seeds. betaGlu I gene expression is associated spatially with the inner testa and temporally with the promotion of testa rupture. Local elevation in moisture content seems to permit local, low-level betaGlu I gene transcription and translation in the maternal tissues of air-dry, low-hydrated seeds. De novo gene expression is therefore proposed to be a novel molecular mechanism for the release of coat-imposed dormancy during oilseed after-ripening.
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