Journal
ANALYTICAL SCIENCES
Volume 31, Issue 4, Pages 307-313Publisher
JAPAN SOC ANALYTICAL CHEMISTRY
DOI: 10.2116/analsci.31.307
Keywords
Two-photon excitation microscopy; multi-point laser scanning method; confocal microscopy; Yb-based laser
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Funding
- JSPS KAKENHI of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [25840044, 25560411, 22300131, 22113005, 26242082]
- Okazaki Institute for Integrative Bioscience
- National Institute for Basic Biology
- Nano-Macro Materials, Devices and System Research Alliance (MEXT)
- Network Joint Research Center for Materials and Devices (MEXT)
- Grants-in-Aid for Scientific Research [15H05953, 25840044, 26242082, 25560411, 22113005, 22300131] Funding Source: KAKEN
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The temporal resolution of a two-photon excitation laser scanning microscopy (TPLSM) system is limited by the excitation laser beam's scanning speed. To improve the temporal resolution, the TPLSM system is equipped with a spinning-disk confocal scanning unit. However, the insufficient energy of a conventional Ti:sapphire laser source restricts the field of view (FOV) for TPLSM images to a narrow region. Therefore, we introduced a high-peak-power Yb-based laser in order to enlarge the FOV. This system provided three-dimensional imaging of a sufficiently deep and wide region of fixed mouse brain slices, clear four-dimensional imaging of actin dynamics in live mammalian cells and microtubule dynamics during mitosis and cytokinesis in live plant cells.
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