3.8 Article

Locus effects and sources of error in noninvasive genotyping

Journal

MOLECULAR ECOLOGY NOTES
Volume 5, Issue 3, Pages 680-683

Publisher

BLACKWELL PUBLISHING
DOI: 10.1111/j.1471-8286.2005.01002.x

Keywords

allelic dropout; faecal DNA; noninvasive genotyping; polymerase slippage; contamination

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In spite of more than a decade of research on noninvasive genetic sampling, the low quality and quantity of DNA in noninvasive studies continue to plague researchers. Effects of locus size on error have been documented but are still poorly understood. Further, sources of error other than allelic dropout have been described but are often not well quantified. Here we analyse the effects of locus size on allelic dropout, amplification success and error rates in noninvasive genotyping studies of three species, and quantify error other than allelic dropout.

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