Development and validation of a methodology for the determination of pesticides in water by SPME-LC/DAD

This study develops a method for solid-phase microextraction (SPME) coupled with high performance liquid chromatography with diode array detector (HPLC-DAD) of two pesticides widely used in sugar cane culture, tebuthiuron and diuron. The SPME-HPLC coupling was made by a home made interface. Parameters affecting the sorption and desorption of analyte, including sampling time, fiber type, stirring rate, pH, ionic strength, temperature, mobile phase composition, and desorption time were evaluated. The best conditions were obtained by a polyacrilate fiber, higher temperatures, sampling time of 50 minutes, and desorption time of 15 minutes.