Journal
FRONTIERS IN BIOSCIENCE-LANDMARK
Volume 10, Issue -, Pages 931-939Publisher
FRONTIERS IN BIOSCIENCE INC
DOI: 10.2741/1587
Keywords
thiol sensors; carbon nanotubes; PQQ; amperometry; cyclic voltammetry
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Funding
- NCRR NIH HHS [1G12RR12459-01] Funding Source: Medline
- NIGMS NIH HHS [S06GM008047] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [R43RR012459] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [S06GM008047] Funding Source: NIH RePORTER
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The electrocatalytic oxidation of thiols has been observed at a glassy carbon ( GC) electrode coated with a single-wall carbon nanotube (SWNT) film. Fourteen thiols including L-cysteine (CySH) and glutathione were tested using the SWNT/GC electrode, and the cyclic voltammetry ( CV) showed that each thiol was oxidized at much less positive potential than those at other electrodes such as bare GC and diamond electrodes. The SWNT/GC electrode was also modified with pyrroloquinoline quinone (PQQ) which showed a further improvement of the catalytic behavior of the SWNT/GC electrode: e. g. the oxidation peak current of CySH was observed at 0.27 V vs. Ag/AgCl in pH 7.5 phosphate buffer. The amperometic responses at these electrodes showed a linear relationship with the substrate concentration in a 10(-6)- 10(-3) M range and 10(-6)- 10(-7) M detection limits for several thiols including CySH, L-homocysteine, N-acetyl-L-cysteine, L-penicillamine and glutathione. These electrodes show a response time of 2-3 s and storage stabilities over 3 weeks. A PQQ/SWNT/GC electrode has been successfully applied for the assay of both L-cysteine and N-acetyl-L-cysteine in the dietary supplement.
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