Journal
CELL BIOLOGY INTERNATIONAL
Volume 29, Issue 1, Pages 65-70Publisher
WILEY
DOI: 10.1016/j.cellbi.2004.11.014
Keywords
fluorescence microscopy; Aox3; pexophagy; Yarrowia lipolytica
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We constructed the fusion of peroxisomal acyl-CoA oxidase 3 and the enhanced yellow fluorescent protein (EYFP) for fluorescent labeling of Yarrowia lipolytica peroxisomes. Using the spectral overlap between EYFP and FM4-64, we developed a procedure for simultaneous observation of Y. lipolytica peroxisomes and vacuoles with the single fluorescein isothiocyanate filter set. Using this procedure we were able to follow the Y. lipolytica peroxisome-vacuole dynamics under pexophagy conditions and show that Y. lipolytica peroxisomes are degraded in the vacuoles by a macropexophagic mechanism. (C) 2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
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