Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 28, Issue 15, Pages 2297-2314Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826070500187491
Keywords
thin layer chromatography; biological applications; amino acids; carbohydrates; drugs; lipids; pigments
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This article reviews the use of thin layer chromatography (TLC) and high performance thin layer chromatography for the analysis of biological samples of particular interest to biologists, biochemists, hematologists, immunologists, medical diagnosticians, and molecular biologists. Determinations of amino acids, drugs, carbohydrates, lipids, toxins, vitamins, indoles, antibiotics, peptides, pigments, phenols, bile acids, and coumarins in sample matrices such as blood, urine, feces, saliva, cerebrospinal fluid, body tissues, and other biologics are considered. The review discusses the advantages of using modem TLC for biological applications and summarizes important information on stationary and mobile phases and methods used for application of standards and samples, plate development, and zone detection, identification, and quantification.
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