Journal
ACTA PHYSIOLOGIAE PLANTARUM
Volume 27, Issue 4B, Pages 675-685Publisher
POLISH ACAD SCIENCES DEPT PLANT PHYSIOLOGY
DOI: 10.1007/s11738-005-0071-x
Keywords
androgenesis; anther culture; doubled haploids; Solanum lycopersicum; microspore development
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The generation of homozygous doubled haploid lines through induction of androgenesis is a promising alternative to the classical inbreeding and selection programs. However, this technology is poorly developed in tomato, where doubled haploid tomato plants have only been obtained through anther culture. Despite the fact that anther culture is routinely used in a number of economically interesting crops, there are still many drawbacks that prevent tomato breeders from adopting this technique, and improvements in methodology are required. One key issue is the correct identification of the optimal stage for anther excision and Culture. In this paper we characterise in vivo microsporogenesis in tomato, defining the different microspore stages and relating them to the length of the donor flower bud. In parallel, we cultured anthers of these stages to obtain embryogenic callus, and followed the microscopic development of the callus contained within the anther. Our data suggest that the stage with the highest response, in terms of callus generation, is meiosis. In particular, we propose the window from metaphase I to telophase II, including tetrad cellalarisation. as the timeframe where induction can be accomplished in tomato anther cultures.
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