Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 28, Issue 7-8, Pages 1253-1263Publisher
TAYLOR & FRANCIS INC
DOI: 10.1081/JLC-200053046
Keywords
UPLC; ultra performance liquid chromatography
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Ultra performance liquid chromatography (TM) (UPLC) takes advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. Using sub-2 mu m particles and mobile phases at high linear velocities, and instrumentation that operates at higher pressures than those used in HPLC, dramatic increases in resolution, sensitivity, and speed of analysis can be obtained. This new category of analytical separation science retains the practicality and principles of HPLC while creating a step-function improvement in chromatographic performance. This review introduces the theory of UPLC, and summarizes some of the most recent work in the field.
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