Journal
NEUROBIOLOGY OF DISEASE
Volume 23, Issue 3, Pages 637-643Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.nbd.2006.05.006
Keywords
apoptosis; cooling; dendrites; epilepsy; Green Fluorescent Protein; hypothermia; dendritic spines; stroke; 2-photon microscope; thermoelectric device
Categories
Funding
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R37DK019645] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R21NS045652, P01NS032636, R21NS049565, R01NS042936] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE ON AGING [R01AG010299] Funding Source: NIH RePORTER
- NIA NIH HHS [R01 AG010299] Funding Source: Medline
- NIDDK NIH HHS [R37 DK019645] Funding Source: Medline
- NINDS NIH HHS [R21 NS045652, R21 NS049565, R01 NS42936, P01 NS32636] Funding Source: Medline
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Cooling is a potential treatment for several neurological diseases. We have examined rodent and cat neocortex, cooled to 5 and 3 degrees C, respectively, to identify a lower limit for safely cooling brain. Rat neocortex, intermittently cooled with a thermoelectric device for 2 h, showed no signs of neuronal injury after cresyl violet or TUNEL staining. Neurons were also preserved in cat cortex cooled for up to 2 h daily for 10 months. Cooled rat and cat cortex showed glial proliferation, but this was also observed in sham-operated rat cortex. When hippocampal slices from mice expressing the Green Fluorescent Protein (GFP) in neurons were cooled to 5 degrees C, but not higher temperatures, we saw reversible dendritic beading and spine loss after 15-30 min. While there may be biochemical and functional alterations in brain cooled as low as 5 degrees C, the neuropathological consequences of brain cooling appear to be insignificant. (c) 2006 Elsevier Inc. All rights reserved.
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