Journal
JOURNAL OF MICROBIOLOGICAL METHODS
Volume 66, Issue 3, Pages 479-485Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.mimet.2006.01.012
Keywords
sterilization; spores; supercritical carbon dioxide
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Funding
- NIBIB NIH HHS [R01 EB 55201] Funding Source: Medline
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Supercritical carbon dioxide (SC CO2) has been evaluated as a new sterilization technology. Results are presented on killing of B. punfilus spores using SC CO2 containing trace levels of additives. Complete killing was achieved with 200 part per million (ppm) hydrogen peroxide in SC CO2 at 60 degrees C, 27.5 MPa. Addition of water to SC CO2 resulted in greater than three-log killing, but this is insufficient to claim sterilization. Neither ethanol nor isopropanol when added to SC CO2 affected killing. (c) 2006 Elsevier B.V. All rights reserved.
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