Journal
LETTERS IN APPLIED MICROBIOLOGY
Volume 43, Issue 3, Pages 331-335Publisher
WILEY
DOI: 10.1111/j.1472-765X.2006.01947.x
Keywords
Beauveria bassiana; conidial production; conidial thermotolerance; medium components; microbial control; optimized culture conditions
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Aims: To produce more thermotolerable conidia of Beauveria bassiana, a well-known fungal biocontrol agent, by optimizing the medium components and culture conditions. Methods and Results: The conidia produced on media including 0.5-6% glucose, sucrose or starch as carbon source and 50-300-mu g ml(-1) Cu2+, Zn2+, Mn2+ or Fe3+ as additive to Sabouraud dextrose medium at 15-30 degrees C, pH 4-8 or KCl-adjusted water availabilities were exposed to 30-min wet heat stress at 48 degrees C. The medium components for conidial production with greatly enhanced thermotolerance included 4% glucose as optimum or 1% starch as alternative for the carbon source and <= 50-mu g ml(-1) Mn2+ for the metal additive. The culture conditions were optimized as 25 degrees C and pH 5-6. Conidial thermotolerance decreased remarkably when sucrose and Fe3+ or Cu2+ were used in the cultures, but altered slightly when 50-200-mu g ml(-1) Zn2+ were included. Conclusions: The tolerance of B. bassiana conidia to the thermal stress was significantly affected by the medium composition and culture conditions under which the conidia were produced. Significance and Impact of Study: Proper treatment of small grains as mass production substrates for more glucose release and supplement of glucose or <= 50-mu g ml(-1) Mn2+ are possible means to enhancing conidial thermotolerance and field persistence for improved insect control.
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