Journal
JOURNAL OF MAGNETIC RESONANCE
Volume 178, Issue 1, Pages 56-64Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jmr.2005.08.011
Keywords
protonless NMR; C-13 NMR; C-13-direct detection; sequential assignment; protein structure
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Carbon-13 direct detection NMR methods are feasible thanks to the improvements in probehead technology and to the development of new NMR experiments. We present here a complete set of experiments, based on C' direct detection, developed to perform protein complete assignment of backbone and side-chains (except for aromatic rings). This strategy offers alternative solutions for demanding situations (paramagnetic and/or large molecules) and can be useful in general in conjunction with conventional experiments. (c) 2005 Elsevier Inc. All rights reserved.
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