Journal
BIOPOLYMERS
Volume 84, Issue 3, Pages 340-347Publisher
WILEY
DOI: 10.1002/bip.20482
Keywords
coupling reagents; model peptide sequences; reagents; plate-based multiple parallel centrifugation synthesizer; difficult sequences; reagent stability; mixed coupling reagents
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Ten different coupling reagents and their combinations were tested in parallel in the synthesis of four model peptide sequences. Significant differences were found between uronium and phosphonium salt-based reagents and carbodiimide. Diisopropylcarbodiimide was identified as an optimal reagent based on the purity of the product, stability of the reagent, and convenience of handling on plate-based multiple parallel centrifugation synthesizer. (c) 2006 Wiley periodicals, Inc.
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