Journal
ANALYTICAL LETTERS
Volume 39, Issue 11, Pages 2227-2233Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/00032710600751016
Keywords
creatine; creatinine; amperometric biosensors; creatininase; creatinase; sorcosine oxidase
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In order to determine creatine and creatinine, amperometric diamond paste biosensors were proposed. A bienzymatic biosensor based on creatinase and sarcosine oxidase was used for the assay of creatine and a trienzymatic biosensor based on creatinase, sarcosine oxidase, and creatininase was proposed for the assay of creatinine. The linear concentration ranges are of fmol/L magnitude order, with very low limits of detection. The biosensors proved to be highly reliable for determination of creatine and creatinine as raw materials in pharmaceutical formulations as well as in serum samples.
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