Journal
GENOME BIOLOGY
Volume 7, Issue 8, Pages -Publisher
BMC
DOI: 10.1186/gb-2006-7-8-r71
Keywords
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Funding
- NHGRI NIH HHS [HG002668, R01 HG002668-03, R01 HG002668] Funding Source: Medline
- NICHD NIH HHS [R01 HD033472, 2R01 HD33472] Funding Source: Medline
- NIDDK NIH HHS [DK070813, K25 DK070813] Funding Source: Medline
- NIMH NIH HHS [R01 MH059942, 5R01 MH59942] Funding Source: Medline
- Wellcome Trust Funding Source: Medline
- Medical Research Council [G0300723B] Funding Source: researchfish
- EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT [R01HD033472] Funding Source: NIH RePORTER
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG002668] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [K25DK070813] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R01MH059942] Funding Source: NIH RePORTER
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We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
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