3.9 Article

Heterologous expression of an Entamoeba histolytica chitin synthase in Saccharomyces cerevisiae

Journal

EUKARYOTIC CELL
Volume 5, Issue 1, Pages 203-206

Publisher

AMER SOC MICROBIOLOGY
DOI: 10.1128/EC.5.1.203-206.2006

Keywords

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Funding

  1. NIAID NIH HHS [R01 AI044070, AI44070] Funding Source: Medline
  2. NIGMS NIH HHS [GM31318, R01 GM031318] Funding Source: Medline
  3. NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI044070] Funding Source: NIH RePORTER
  4. NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R37GM031318, R01GM031318] Funding Source: NIH RePORTER

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Chitin in the cyst wall of Entamoeba histolytica is made by two chitin synthases (Chs), one of which is unique (EhCHS-1) and one of which resembles those of insects and nematodes (EhCHS-2). EhCHS-1 is deposited chitin in the lateral wall of transformed Saccharomyces cerevisiae Chs mutants, independent of accessory proteins (Chs4p to Chs7p) required by yeast Chs3p.

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