Journal
ANNALS OF BIOMEDICAL ENGINEERING
Volume 34, Issue 9, Pages 1429-1441Publisher
SPRINGER
DOI: 10.1007/s10439-006-9156-y
Keywords
photocrosslinking; hydrogels; layered manufacturing; rapid prototyping; tissue engineering; scaffold fabrication; fibroblasts
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Funding
- NATIONAL CENTER FOR RESEARCH RESOURCES [G12RR008124] Funding Source: NIH RePORTER
- NCRR NIH HHS [G12 RR08124] Funding Source: Medline
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Stereolithography (SL) was used to fabricate complex 3-D poly(ethylene glycol) (PEG) hydrogels. Photopolymerization experiments were performed to characterize the solutions for use in SL, where the crosslinked depth (or hydrogel thickness) was measured at different laser energies and photoinitiator (PI) concentrations for two concentrations of PEG-dimethacrylate in solution (20% and 30% (w/v)). Hydrogel thickness was a strong function of PEG concentration, PI type and concentration, and energy dosage, and these results were utilized to successfully fabricate complex hydrogel structures using SL, including structures with internal channels of various orientations and multi-material structures. Additionally, human dermal fibroblasts were encapsulated in bioactive PEG photocrosslinked in SL. Cell viability was at least 87% at 2 and 24 h following fabrication. The results presented here indicate that the use of SL and photocrosslinkable biomaterials, such as photocrosslinkable PEG, appears feasible for fabricating complex bioactive scaffolds with living cells for a variety of important tissue engineering applications.
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