Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 50, Issue 1, Pages 36-42Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2006.03.020
Keywords
bovine serum albumin; N-isopropylacrylamide; acrylic acid; microgel; volume phase transition
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Monodispersed poly(N-isopropylacrylamide-co-acrylic acid) (PNIPAM/AAc) microgels with various contents of acrylic acid (AAc) and crosslinker NAP-methylenebisacrylamide (MBAAm) were synthesized by surfactant-free emulsion polymerization. The microgels exhibited affinity of binding bovine serum albumin (BSA) with increase of AAc contents. The adsorption amount of BSA was strongly dependent on suspension pH. The maximum adsorption was observed at around pH 4.0, while both low pH and basic conditions induced the rather low adsorption. In addition, AFM images showed that the microgel particles underwent bridging aggregation by loading BSA. The resultant BSA-microgel particles became more hydrophobic after loading BSA. Importantly, the adsorption of BSA onto PNIPAM/AAc microgel was found to alter the swelling/deswelling behavior of the resultant microgel suspensions. With the increase of BSA concentration, the volume phase transition temperature of BSA-microgel particles shifted to the lower temperature. (C) 2006 Elsevier B.V. All rights reserved.
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