Journal
STRUCTURE
Volume 14, Issue 6, Pages 1039-1048Publisher
CELL PRESS
DOI: 10.1016/j.str.2006.03.018
Keywords
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Funding
- Wellcome Trust Funding Source: Medline
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Bacteriophage phi 6 is an enveloped dsRNA virus with a segmented genome. phi 6 specifically packages one copy of each of its three genome segments into a pre-assembled polymerase complex. This leads to expansion of the polymerase complex, minus and plus strand RNA synthesis, and assembly of the nucleocapsid. The phi 6 in vitro assembly and packaging system is a valuable model for dsRNA virus replication. The structure of the nucleocapsid at 7.5 angstrom resolution presented here reveals the secondary structure of the two major capsid proteins. Asymmetric P1 dimers; organize as an inner T=1 shell, and P8 trimers organize as an outer T=13 laevo shell. The organization of the P1 molecules in the unexpanded and expanded polymerase complex suggests that the expansion is accomplished by rigid body movements of the P1 monomers. This leads to exposure of new potential RNA binding surfaces to control the sequential packaging of the genome segments.
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