Journal
GENOMICS
Volume 87, Issue 1, Pages 181-190Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2005.09.005
Keywords
androgen receptor; BAC library; bird; genomics; zebra finch
Funding
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [U01HG002525] Funding Source: NIH RePORTER
- NHGRI NIH HHS [U1HG02525A] Funding Source: Medline
- NIDCD NIH HHS [DC000217] Funding Source: Medline
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The zebra finch (Taeniopygia guttata) is an important model organism for studying behavior, neuroscience, avian biology, and evolution. To support the study of its genome, we constructed a BAC library (TG_Ba) using DNA from livers of females. The BAC library consists of 147,456 clones with 98% containing inserts of an average size of 134 kb and represents 15.5 haploid genome equivalents. By sequencing a whole BAC, a full-length androgen receptor open reading frame was identified, the first in an avian species. Comparison of BAC end sequences and the whole BAC sequence with the chicken genome draft sequence showed a high degree of conserved synteny between the zebra finch and the chicken genome. (c) 2005 Elsevier Inc. All rights reserved.
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