Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 22, Issue 1, Pages 51-57Publisher
SPRINGER
DOI: 10.1007/s11274-005-5773-0
Keywords
biodegradation; fungi; P(3HB) depolymerase; P(3HB-co-3HV)-degrading enzyme
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Poly(3-hydroxybutyrate) [P(3HB)] depolymerase was purified from a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)]-degrading fungus, Paecilomyces lilacinus F4-5 by hydrophobic and ion exchange column chromatography, and showed a molecular mass of 45 kDa. The optimum temperature and pH of the P(3HB) depolymerase were 50 degrees C and 7.0, respectively. The enzyme was stable for at least 30 min at temperatures below 40 degrees C, while the activity abruptly decreased over 55 degrees C. Enzymatic P(3HB-co-3HV) degradation showed a similar degradation pattern to that of film overlaid by fungal hyphae. It reflects that the fungal degradation of P(3HB-co-3HV) in soil is mainly caused by extracellular depolymerases.
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