Journal
JOURNAL OF STRUCTURAL BIOLOGY
Volume 153, Issue 1, Pages 55-63Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jsb.2005.10.005
Keywords
electron tomography; 3-D reconstruction algorithm; double-tilt; dual-axis; iterative reconstruction; SIRT
Funding
- Engineering and Physical Sciences Research Council [GR/R30457/01] Funding Source: researchfish
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A new algorithm for computing electron microscopy tomograms which combines iterative methods with dual-axis geometry is presented. Initial modelling using test data shows several improvements over both the weighted back-projection and simultaneous iterative reconstruction technique methods, with increased stability and tomogram fidelity under high-noise conditions. Preliminary experimental dual-axis reconstructions confirm the viability of the new algorithm. (C) 2005 Elsevier Inc. All rights reserved.
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