Sensitive determination of synephrine by flow-injection chemiluminescence

It was found that light emission produced by the oxidation of luminol by potassium ferricyanide in basic medium was enhanced by synephrine, an anti-obesity drug. The optimum conditions for this chemiluminescent reaction were studied in detail in a flow injection system and employed in a new, simple and rapid method for the determination of synephrine. A mechanism for this reaction is proposed, based on the chemiluminescence reaction spectra. In the optimum conditions, CL intensity is proportional to concentration of synephrine in the 0.008-1 mu g/mL range. The limit of detection is 1.6 ng/mL for synephrine (3 sigma), and the relative standard deviation (n = 11) is 2.6% for 0.5 mu g/mL synephrine. The method was applied to the determination of synephrine in herbal products, citrus fruit and biological fluids. The recoveries were satisfactory (90-102%). The results given by the proposed method are in good agreement with those given by HPLC-UV. Copyright (C) 2005 John Wiley & Sons, Ltd.