Journal
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
Volume 25, Issue 4-6, Pages 369-382Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15257770600683953
Keywords
in vitro selection; microchip electrophoresis; RNA aptamer; SYBR gold
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DNA and RNA can be separated by microchip electrophoresis (ME) and detected using an intercalating fluorescent dye. The advantages of this method are short sensing times (< 3 min), avoidance of a radioisotope labeling detection system, relatively low costs, and reduced labor intensity. In the present study, RNA aptamer-protein or -peptide interactions were analyzed using ME and the regression of free aptamers corresponding to unbound RNA was detected as the target protein or peptide increased in a dose-dependent manner. Our results demonstrate the applicability of this method to simple, rapid ligand screening in the interactions between oligonucleotides and their targets.
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