Acteoside and martynoside exhibit estrogenic/antiestrogenic properties

Acteoside and martynoside are plant phenylpropanoid glycosides exhibiting anticancer, cytotoxic and antimetastatic activities. We investigated their potential to activate estrogen receptor isoforms ER alpha and ER beta in HeLa cells transfected with an estrogen response element (ERE)-driven luciferase (Luc) reporter gene and an ER alpha or ER beta expression vector. Their estrogenic/antiestrogenic effects were also assessed in breast cancer cells (MCF7), endometrial cancer cells (Ishikawa) and osteoblasts (KS483), by measuring IGFBP3 levels, cell viability and number of mineralized nodules, respectively, seeking for a natural selective estrogen receptor modulator (SERM). Acteoside and martynoside antagonized both ERa and ER beta (p < 0.001), whereas they reversed the effect of E-2 mainly via ER alpha (p < 0.001). Martynoside was a potent antiestrogen in MCF-7 cells, increasing, like ICI182780, IGFBP3 levels via the ER-pathway. In osteoblasts, martynoside induced nodule mineralization, which was abolished by ICI182780, implicating an ER-mediated mechanism. Furthermore, its antiproliferative effect on endometrial cells suggests that martynoside may be an important natural SERM. Acteoside was an antiestrogen in breast cancer cells and ostcoblasts, without any effect on endometrial cells. Our study suggests that the nature is rich in selective ER alpha and ER beta ligands, the discovery of which may lead to the development of novel neutraceutical agents. (c) 2005 Elsevier Ltd. All rights reserved.