Journal
PLANT BREEDING
Volume 125, Issue 3, Pages 254-258Publisher
WILEY
DOI: 10.1111/j.1439-0523.2006.01221.x
Keywords
Setaria viridis; Setaria italica; alpha 2-tubulin mutation; distorted segregation; trifluralin herbicide resistance
Ask authors/readers for more resources
Inheritance of trifluralin herbicide resistance was investigated for phenotype and genotype (molecular identification) in 12 segregating populations derived from crosses between resistant green foxtail (Setaria viridis) and foxtail millet cultivars (S. italica). Combining a herbicide bioassay and a bidirectional allele-specific polymerase chain reaction (PCR) assay, the trifluralin resistance in foxtail millet was proved to be conferred by a recessive alpha 2-tubulin gene mutation at Ile-239, the allele transferred by green foxtail. However, a distorted segregation ratio in F-2 populations was confirmed, with 16.9% resistant plants on average instead of the 25% expected for a single gene recessive mutation. This was not due to non-germinating seed and the distortion was also observed in the next generations, indicating a heritable phenomenon. After further crosses and self-fertilization, however, more advanced generations did not show the same pattern of skewed inheritance as in the early generations. These results suggest the possibility of linkage between the alpha 2-tubulin gene and a modifier gene to explain the distorted segregation, which might be broken by crossing over.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available