Journal
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
Volume 30, Issue 11, Pages 1060-1069Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2006.01.001
Keywords
antimicrobial peptide; beta-defensin; lung; preterm infant; sheep; surfactant protein; toll-like receptor
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Funding
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI062787, K08AI055499] Funding Source: NIH RePORTER
- NIAID NIH HHS [K08 AI055499, K08 AI055499-03, R01 AI062787-05, K08AI055499-03, R01 AI062787] Funding Source: Medline
- PHS HHS [R01 RAI062787A] Funding Source: Medline
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Preterm infants experience enhanced susceptibility and severity to respiratory syncytial virus (RSV) infection. Terminal airway epithelium is an important site of RSV infection and the extent of local innate immune gene expression is poorly understood. In this study, expression of surfactant proteins A and D (SP-AD), sheep beta defensin I (SBD1), and toll-like receptor 4 (TLR4) mRNA were determined in whole lung homogenates from lambs. SP-AD and TLR4 mRNA expression increased (p < 0.05) from late gestation to term birth. In addition, gene expression of LCM-retrieved type 11 pneumocytes (CD208+), adjacent epithelium (CD208-) and bronchial epithelium demonstrated that bronchiole-alveolar junction epithelium (combined CD208+/-) had significant (p < 0.05) developmental increases in SP-AD, SBD1 and TLR4 mRNA, whereas CD208+ cells had statistically significant increases only with SP-A mRNA. Using immunofluorescence, SP-AD antigen distribution and intensity were also greater with developmental age. These studies show reduced SBD1, SP-AD, and TLR4 expression in the preterm lung and this may underlie enhanced RSV susceptibility. (c) 2006 Elsevier Ltd. All rights reserved.
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