Journal
SYSTEMATIC AND APPLIED MICROBIOLOGY
Volume 29, Issue 1, Pages 45-48Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.syapm.2005.06.002
Keywords
Chromobacterium violaceum; multiplex PCR; type-III secretion system
Categories
Ask authors/readers for more resources
Based on the recently completed genomic sequence of Chromobacterium violaceum American Type Culture Collection (ATCC) 12472 a multiplex PCR assay targeting the prgI, spaO, invG, and sipB genes of the Salmonella SPI-I homologue type-III secretion system was developed. PCR products of 255 bp (prgI), 749 bp (spaO), 1685 bp (invG), and 1752 bp (sipB) were successfully amplified simultaneously in a single reaction with all Chr. violaceum strains investigated whereas other bacteria tested negative. The detection limit for pure cultures in multiplex PCR analysis was 100 CFU. The developed assay significantly improves rapid identification of Chr. violaceum and allows its differentiation from closely related organisms. (c) 2005 Elsevier GmbH. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available