Journal
PHYTOCHEMICAL ANALYSIS
Volume 17, Issue 1, Pages 15-19Publisher
WILEY
DOI: 10.1002/pca.874
Keywords
bioautography; xanthine oxidase; superoxide radical; plant extracts
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A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated. Copyright (c) 2005 John Wiley & Sons, Ltd.
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