Journal
MICROBIOLOGICAL RESEARCH
Volume 161, Issue 2, Pages 158-163Publisher
ELSEVIER GMBH, URBAN & FISCHER VERLAG
DOI: 10.1016/j.micres.2005.07.007
Keywords
human herpes virus 6 (HHV-6); PCR; real-time PCR
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A comparative study was conducted of a novel real-time quantitative PCR test (LightCycter System) with FastStart DNA Master(PLUS) SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection Limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at tower cost. (C) 2005 Elsevier GmbH. All rights reserved.
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