Journal
ANNUAL REVIEW OF PLANT BIOLOGY
Volume 57, Issue -, Pages 79-107Publisher
ANNUAL REVIEWS
DOI: 10.1146/annurev.arplant.57.032905.105239
Keywords
confocal scanning laser microscopy; green fluorescent protein; biosensors; cameleon; live-cell imaging
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Funding
- Biotechnology and Biological Sciences Research Council [BBS/B/03904, REI20537] Funding Source: Medline
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A substantial number of elegant experimental approaches have been developed to image the distribution and dynamics of DNA, mRNA, proteins, organelles, metabolites, and ions in living plant cells. Although the human brain can rapidly assimilate visual information, particularly when presented as animations and movies, it is much more challenging to condense the phenomenal amount of data present in three-, four-, or even five-dimensional images into statistically useful measurements. This review explores a range of in vivo fluorescence imaging applications in plants, with particular emphasis on where quantitative techniques are beginning to emerge.
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