Journal
JOURNAL OF ENDOCRINOLOGY
Volume 228, Issue 1, Pages 39-48Publisher
BIOSCIENTIFICA LTD
DOI: 10.1530/JOE-15-0247
Keywords
BB2; neuromedin C; forskolin/IBMX; perfused mouse intestine; incretin hormones
Categories
Funding
- Novo Nordisk Foundation Center for Basic Metabolic Research
- European Union's Seventh Framework Programme [8266408]
- Danish Diabetes Academy - Novo Nordisk Foundation
- Lundbeck Foundation
- Wellcome Trust [WT106262/Z/14/Z, WT106263/Z/14/Z]
- MRC [MRC_MC_UU_12012/3]
- Lundbeck Foundation [R180-2014-2868] Funding Source: researchfish
- NNF Center for Basic Metabolic Research [Schwartz Group, Holst Group] Funding Source: researchfish
- Novo Nordisk Fonden [NNF15OC0016574, NNF14OC0010889] Funding Source: researchfish
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The incretin hormones glucagon-like peptide-1 (GLP1) and glucose-dependent insulinotropic polypeptide (GIP) are secreted from intestinal endocrine cells, the so-called L- and K-cells. The cells are derived from a common precursor and are highly related, and co-expression of the two hormones in so-called L/K-cells has been reported. To investigate the relationship between the GLP1- and GIP-producing cells more closely, we generated a transgenic mouse model expressing a fluorescent marker in GIP-positive cells. In combination with a mouse strain with fluorescent GLP1 cells, we were able to estimate the overlap between the two cell types. Furthermore, we used primary cultured intestinal cells and isolated perfused mouse intestine to measure the secretion of GIP and GLP1 in response to different stimuli. Overlapping GLP1 and GIP cells were rare (similar to 5%). KCI, glucose and forskolin + IBMX increased the secretion of both GLP1 and GIP, whereas bombesin/neuromedin C only stimulated GLP1 secretion. Expression analysis showed high expression of the bombesin 2 receptor in GLP1 positive cells, but no expression in GIP-positive cells. These data indicate both expressional and functional differences between the GLP1-producing 'L-cell' and the GIP-producing 'K-cell'.
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