Journal
CANCER RESEARCH
Volume 66, Issue 1, Pages 134-142Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/0008-5472.CAN-05-2982
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Funding
- NCI NIH HHS [P30 CA47904, CA098675] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R01CA098675, P30CA047904] Funding Source: NIH RePORTER
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Rev3L encodes the catalytic subunit of DNA polymerase C (pol zeta) in mammalian cells. In yeast, pol C helps cells bypass sites of DNA damage that can block replication enzymes. Targeted disruption of the mouse Rev3L gene causes lethality midway through embryonic gestation, and Rev3L(-/-) mouse embryonic fibroblasts (MEFs) remain in a quiescent state in culture. This suggests that pol C may be necessary for tolerance of endogenous DNA damage during normal cell growth. We report the generation of mitotically active Rev3L(-/-) MEFs on a p53(-/-) genetic background. Rev3L null MEFs exhibited striking chromosomal instability, with a large increase in translocation frequency. Many complex genetic aberrations were found only in Rev3L null cells. Rev3L null cells had increased chromosome numbers, most commonly near pentaploid, and double minute chromosomes were frequently found. This chromosomal instability associated with loss of a DNA polymerase activity in mammalian cells is similar to the instability associated with loss of homologous recombination capacity. Rev3L null MEFs were also moderately sensitive to mitomycin C, methyl methanesulfonate, and UV and gamma-radiation, indicating that mammalian pol zeta helps cells tolerate diverse types of DNA damage. The increased occurrence of chromosomal translocations in Rev3L(-/-) MEFs suggests that loss of Rev3L expression could contribute to genome instability during neoplastic transformation and progression.
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