Journal
JOURNAL OF NEUROCHEMISTRY
Volume 96, Issue 1, Pages 195-203Publisher
WILEY
DOI: 10.1111/j.1471-4159.2005.03531.x
Keywords
BDNF; Ca2+/calmodulin-dependent protein kinase II; dopamine; substantia nigra; tyrosine hydroxylase
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Ca2+/calmodulin-dependent protein kinase (CaMK)II is highly expressed in the CNS and mediates activity-dependent neuronal plasticity. Four CaMKII isoforms, alpha, beta, gamma and delta, have a large number of splicing variants. Here we identified isoforms of CaMKII in the rat substantia nigra (SN). Northern blot and RT-PCR analyses revealed that the gamma and delta isoform mRNAs with several splicing variants were predominantly expressed in SN. Immunoblot analysis indicated that the major isoforms were gamma A, gamma C, delta 1 and delta 3. An immunohistochemical study also confirmed the preferential localization of gamma and delta isoforms in SN dopaminergic neurons. In dopaminergic neurons, immunoreactivity against anti-CaMKII delta 1-4 antibody was detected in both nucleus and cytoplasm, in contrast to the predominant expression of gamma isoforms in the cytoplasm. Furthermore, we showed expression of brain-derived neurotrophic factor (BDNF) mRNAs with exons II and IV in SN. Taken together with our previous observations, the results suggest that the CaMKII delta 3 isoform is involved in the expression of BDNF in the SN.
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