Journal
NUCLEIC ACIDS RESEARCH
Volume 34, Issue 10, Pages 2878-2886Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkl081
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Modified nucleosides play an important role in structure and function of tRNA. We have determined the solution structure of the anticodon stem-loop (ASL) of initiator tRNA of Schizosaccharomyces pombe. The incorporation of N6-threonylcarbamoyladenosine at the position 31 to the anticodon triplet (t(6)A37) results in the formation of a U-turn motif and enhances stacking interactions within the loop and stem regions (i.e. between A35 and t(6)A37) by bulging out U36. This conformation was not observed in a crystal structure of tRNAi including the same modification in its anticodon loop, nor in the solution structure of the unmodified ASL. A t(6) A modification also occurs in the well studied anti-stem-loop of lystRNA(UUU). A comparison of this stem-loop with our structure demonstrates different effects of the modification depending on the loop sequence.
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