Journal
JOURNAL OF NEUROCHEMISTRY
Volume 96, Issue 1, Pages 82-96Publisher
WILEY
DOI: 10.1111/j.1471-4159.2005.03510.x
Keywords
axotomy; frog; growth factor; mitogen-activated protein kinase; neurotrophin; retinal ganglion cells
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Funding
- NCRR NIH HHS [G12RR-03051] Funding Source: Medline
- PHS HHS [S06 G08224] Funding Source: Medline
- NATIONAL CENTER FOR RESEARCH RESOURCES [G12RR003051] Funding Source: NIH RePORTER
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Application of basic fibroblast growth factor (FGF-2) to the optic nerve after axotomy promotes the survival of retinal ganglion cells (RGCs) in the frog, Rana pipiens. Here we investigate the effects of FGF-2 treatment upon the synthesis of brain-derived neurotrophic factor (BDNF) and its receptor, tyrosine receptor kinase B (TrkB). Axotomy alone increased the amounts of BDNF and TrkB mRNA in RGCs after 1 week and 48 h, respectively; FGF-2 treatment to the nerve accelerated and increased this up-regulation of both. FGF-2 also increased the amounts of phosphorylated cAMP response element binding protein (pCREB) in the retina. Blocking extracellular-regulated kinase (ERK) activation with PD98059 or U0126 prevented the FGF-2-induced up-regulation of BDNF transcription but had no effect on TrkB. However, blocking protein kinase A (PKA) with H89 or Rp-8-Cl-cAMPS reduced the up-regulation of both BDNF and TrkB, and reduced pCREB. In addition, H89 inhibited ERK activation, indicating cross-talk between the pathways. Finally, axonal application of blocking antibody against the FGF receptor 1 (FGFR1) prevented the FGF-2-induced up-regulation of BDNF and TrkB. Our results suggest that FGF-2 acts on RGCs via FGFR1, activating the ERK pathway and CREB to increase BDNF synthesis, and PKA and CREB to increase TrkB synthesis.
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