Journal
MOLECULAR AND CELLULAR BIOLOGY
Volume 26, Issue 1, Pages 293-302Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.26.1.293-302.2006
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NF-kappa B-repressing factor (NRF) is a transcriptional silencer protein that specifically counteracts the basal activity of several NF-kappa B-dependent promoters by direct binding to specific neighboring DNA sequences. In cell culture experiments, the reduction of NRF mRNA leads to a derepression of beta interferon, interleukin-8, and inducible nitric oxide synthase transcription. The X chromosome-located single-copy NRF gene is ubiquitously expressed and encodes a protein of 690 amino acids. The N-terminal part contains a nuclear localization signal, the DNA-binding domain, and the NF-kappa B-repressing domain, while the C-terminal part is responsible for double-stranded RNA binding and nucleolar localization. To study the function of NRF in a systemic context, transgenic mice lacking the NRF gene were created. Against predictions from in vitro experiments, mice with a deletion of the NRF gene are viable and have a phenotype that is indistinguishable from wild-type mice, even after challenge with different pathogens. The data hint towards an unexpected functional redundancy of NRF.
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