Journal
JOURNAL OF BACTERIOLOGY
Volume 188, Issue 1, Pages 343-347Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/JB.188.1.343-347.2006
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Although Nitrosomonas europaea lacks measurable alpha-ketoglutarate dehydrogenase activity, the recent completion of the genome sequence revealed the presence of the genes encoding the enzyme. A knockout mutation was created in the sucA gene encoding the El subunit. Compared to wild-type cells, the mutant strain showed an accelerated loss of ammonia monooxygenase and hydroxylamine oxidoreductase activities upon entering stationary phase. In addition, unlike wild-type cells, the mutant strain showed a marked lag in the ability to resume growth in response to pH adjustments in late stationary phase.
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